25-Hydroxyl Vitamin D Test Kit
Product Description
REAGEN™ The 25-Hydroxyl Vitamin D Test Kit is suitable for the quantitative determination of 25-hydroxy vitamin D in human serum or plasma in vitro.
Procedure Overview
REAGEN™ The concentration of 25-hydroxy vitamin D in human serum was quantitatively determined by using fluorescence immunochromatography and competitive inhibition. The product detects the conjugate of 25-hydroxy vitamin D total antigen and bovine serum albumin,the quality control line is coated with goat anti-mouse IgG, and the fluorescent label tube contains anti-25-hydroxy vitamin D mouse monoclonal antibody-labeled fluorescent microspheres. ball. At the time of detection, if the sample contains 25-hydroxy vitamin D, an immune reaction with the antibody-labeled fluorescent microspheres will occur, thereby immunosuppressing the reaction between the labeled microspheres and the antigen on the detection line, and the fluorescence signal intensity of the detection line will be weakened accordingly. ; Whether or not the sample contains 25-hydroxy vitamin D, the microspheres will form a certain intensity fluorescent band in the quality control area.
The signal intensity of the band is negatively correlated with the concentration of 25-hydroxy vitamin D. The response signal of the quality control line and detection line of the 25-hydroxy vitamin D detection reagent is obtained by an immunofluorescence chromatography analyzer, and the measured signal can be generated through the measurement and analysis of the instrument. Quantitative test results of 25-hydroxy vitamin D in the sample.
Kit Contents, Storage and Shelf Life
Component
Quantity
10 servings / box
20 servings / box
Detection reagent
1 serving × 10
1 serving × 20
Fluorescent reaction solution
400μl / piece × 1 branch
800μl / piece × 1 branch
Precipitation release
400μl / piece × 1 branch
800μl / piece × 1 branch
Display board reaction solution
700μl / piece × 1 branch
1400μl / piece × 1 branch
Sample processing tube
10 pcs / bag × 1 bag
20 pcs / bag × 1 bag
ID card
1 sheet
1 sheet
Store at 2 ℃ ～ 30 ℃, the valid period is 18 months. Please refer to the packaging label for the
production date and valid period.
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Human Serum ELISA Assay 25- Hydroxyl Vitamin D Test Kit Images
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